Research
Nanopore Microscopy for Single-Cell Profiling
The genomics revolution, driven by single-molecule sequencing technologies, has greatly enhanced precision medicine. Similarly, single-cell proteomics and secretomics are poised to revolutionize our understanding of cells, contributing significantly to drug and cell-based therapy development. Currently lacking a gold standard, single-cell proteomics faces challenges in profiling live cells. We aim to enable single-cell profiling for in situ analysis of living cells by employing Scanning Nanopore Microscopy. We perform intracellular and extracellular recording of single-cells to monitor the dynamic process of protein production and secretion, aiming to understand complex biological processes such as immune cells activity and function.
The Exciting World of Single Biomolecule Sensors:
Nanopore-based sensing allows analyses of biomolecules (such as DNA and proteins) with sub-nanometer resolution and without the need for labelling or amplification down to the single-molecule level. Solid-state nanopore sensors have become a common tool for analysis of single biomolecules such as DNA. We’ve developed a technique for fabrication of nanopores with single nanometer precision using ion beam irradiation. Such a platform leads to the development of new generation solid-state nanoporous devices for massively parallel detection of single biomolecules. If you are interested in this project, you can contact me for further discussion.